Plasmid

Part:BBa_K1297000:Design

Designed by: Kyoungwoo Jang   Group: iGEM14_CSIA-SouthKorea   (2014-06-05)


Klebsiella oxytoca Urease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 225
    Illegal XhoI site found at 337
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 452
    Illegal NgoMIV site found at 673
    Illegal NgoMIV site found at 1339
    Illegal NgoMIV site found at 1754
    Illegal NgoMIV site found at 1841
    Illegal NgoMIV site found at 2788
    Illegal NgoMIV site found at 3578
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1900
    Illegal BsaI.rc site found at 148
    Illegal BsaI.rc site found at 3197
    Illegal SapI.rc site found at 3938


Design Notes

A size of the urease is very big (about 5 kb) because this gene has many subunits.


Source

genomic DNA of Klebsiella oxytoca KCTC 1686

References